League One promotion hopefuls Brentford and Leyton Orient both dropped points on Tuesday as Wolves increased their lead at the top.
Second-placed Brentford suffered a comprehensive 3-0 defeat at Rotherham United, but third-placed Orient failed to take advantage as they were held to a 1-1 home draw with Oldham Athletic.
Kieran Agard scored a first-half double to put Rotherham in command, the striker's first coming from the penalty spot as he took his tally for the season to 20 goals.
On loan Newcastle midfielder Haris Vuckic rounded off the win for Rotherham, who are now just four points adrift of third-placed Orient.
Meanwhile, a late Korey Smith equaliser denied Orient all three points against Oldham after defender Scott Cuthbert had given Russell Slade's side the lead.
And Wolves took full advantage of their rivals' slip-ups, as they moved four points clear at the summit after coming out on the right side of a six goal thriller with Colchester United, Kenny Jackett's men claiming a 4-2 win.
Goals from Alex Gilbey and David Wright gave Colchester hope and set up a tense finale after Wolves had surged ahead with three first-half goals from Michael Jacobs, David Edwards and James Henry.
But Nouha Dicko made sure of the points for the hosts in injury time.
At the other end of the table, Notts County moved off the bottom with a 3-1 win at Crewe Alexandra, Ronan Murray scoring a double to help them leapfrog Stevenage, who visit Coventry City on Wednesday.
Fellow strugglers Carlisle United and Shrewsbury Town played out a 0-0 draw, and Tranmere Rovers succumbed to a 2-1 home loss to Swindon Town.
Preston North End overcame Peterborough United 3-1 thanks to a late brace from Joe Garner, but MK Dons' play-off hopes were dealt a blow with a 1-0 reverse at home to Gillingham.
Bristol City were the big winners of the day, surging to a 5-0 victory over Port Vale - who had Carl Dickinson sent off when four goals down - thanks to doubles from Jay Emmanuel-Thomas and Sam Baldock.
Elsewhere, Conor Coady scored twice as Sheffield United beat Crawley Town 2-0, and Craig Westcarr netted both goals in Walsall's 2-0 triumph at Bradford City.
|22||K. Agard||14' 45'|
|Coach: S. Evans|
for B. Pringle 88'
for K. Agard 90+1'
|Kenya 0 Ethiopia 0 (agg 0-2): Hosts sent out of CHAN qualifiers after second penalty miss|
|Dortmund put 17 past Olympians, Bremen hand out thrashing|
|Dinamo Zagreb confirm arrests of coach and club president|
|I didn´t know who Wimbledon guest Klopp was - Brown|
|LeBron James backs Liverpool captain Henderson for FIFA 16 cover.|
|Ramos: I am not negotiating with anyone|
|Valencia complete Mina swoop|
|United States v Japan: US seek final redemption|
|Hanousek consigns Celtic to defeat|
|Roma confirm Dzeko interest|
|Arsenal forward Podolski completes Galatasaray switch|
|Warm Dortmund welcome delights Tuchel|
|Silva vows to fight for Madrid place|
|Mancini hints at Shaqiri and Santon departures|
|Bojan raring to go after return from injury|
|Babel backs Firmino to shine at Liverpool|
|Falcao must wait to impress, warns Chelsea favourite|
|Bartomeu: Deal in place for Turan|
|Parejo re-signs with Valencia|
|MLS Review: Sounders return to winning ways|
|Klinsmann unhappy with USA performance|
|High hopes for Paraguay - Diaz|
|Gareca: More to come from Peru|
|Gerrard had European offers|
|Argentina out to spoil Chile´s party - Martino|
|Sampaoli: Chile won´t change style|
|Di Maria will come good at United - Rojo|
|Peru 2 Paraguay 0: Gareca´s men finish third at Copa|
|United States 4 Guatemala 0: Klinsmann´s men win comfortably|
|´I’d prefer no-one to know my name´ - Bassett|
|Matthews completes Sunderland move|
|Podolski set for Galatasaray medical|
|Gundogan inspires Dortmund victory|
|Inter confirm Montoya capture|
|Murillo joins Inter, as Kuzmanovic and Obi depart|
|Falcao signing not a risk, says former Chelsea star|
|Deeney agrees new Watford deal|
|Confidence and service the key to Falcao success at Chelsea|
|No bids for ´Chelsea target´ Baba, claim Augsburg|
|Derby swoop for Ince|
|Fiorentina consider legal action over Salah ´no-show´|
|Chelsea the team to beat in the Premier League, says Neville|
|Storari exits Juve for Cagliari|
|UEFA removes FFP restrictions on Man City and PSG|
|West Brom hope for new owners within the month|
|Chelsea agree loan deal for Falcao|
|Kuyt eyes trophies on Feyenoord return|
|Chile v Argentina: History beckons ahead of Copa America final|
|Wallace extends Monaco loan|
|Mihajlovic wants to instill fear factor at Milan|
|Mignolet improving at Liverpool - Grobbelaar|
|Mumbai City name Anelka player-manager|
|Olivia the parrot predicts Japan to win Women´s World Cup|
|Lusitans coach blasts Bilic´s ´lack of respect´|
|Sakho eyes more Europa League wins|
|Gareca wants Peru to keep building|
|Chile ´no chance´ if Argentina produce their best - Diaz|
|Ocampos joins Marseille permanently|
|Europa League Review: Cloud-nine Brondby ignite opening round|