Tottenham right-back Kyle Walker has withdrawn from the England squad to face Denmark on Wednesday with a groin injury.
A statement on the club's official website confirmed the news, with Walker having missed Spurs' last four matches with the problem.
Liverpool's Glen Johnson or Manchester United's Chris Smalling now look the most likely candidates to fill the right-back role as England play their final friendly before Roy Hodgson names his 30-man provisional squad for the FIFA World Cup.
Hodgson revealed the news was disappointing if not unexpected, and that Liverpool's Glen Johnson would earn his 50th cap at right-back in Walker's absence.
"We were hoping he would recover and maybe play for Tottenham at weekend but unfortunately he needs another week we think so we've had to let him go," he said.
"We half knew to be honest that he wouldn't be able to make it. Glen Johnson will get his 50th cap."
Johnson’s club-mate and England captain Steven Gerrard praised the 29-year-old ahead of his milestone appearance, insisting there was more to come.
"Glen's a top right-back," Gerrard said. "He's a big athlete, he's been consistent for a long time and I'm sure if he continues to play well he'll have other targets to hit."
|Coach: R. Hodgson|
for A. Cole 46'
for J. Wilshere 59'
for W. Rooney 60'
for J. Henderson 77'
for R. Sterling 86'
for D. Sturridge 87'
for L. Jacobsen 46'
for E. Larsen 46'
for S. Kjær 62'
for C. Sloth 62'
for N. Bendtner 63'
for J. Poulsen 81'
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