Atletico Mineiro begin their Copa Libertadores defence at Zamora on Tuesday, while Santos Laguna meet Arsenal.
Jo's seven goals last campaign led Mineiro to their maiden title, but their form in the league has been questionable early in the season.
Paulo Autuori's men have won just one of their opening four games, and Jo has netted twice already in good signs.
They have been drawn in Group 4 alongside Paraguay's Nacional Asuncion, Venezuela's Zamora and Colombia's Santa Fe.
A trip to an in-form Zamora shapes as being tricky, with the Venezuelans in good form domestically.
Juan Falcon (eight goals), Pedro Ramirez (six), Ricardo Clarke and John Murillo (both five) have been scoring regularly.
Nacional Asuncion visit Santa Fe, also on Tuesday, in the other Group 4 game.
In Group 8, Santos Laguna host Arsenal with both teams looking to find some form early in the competition.
Oribe Peralta shapes as being the main danger to Arsenal with the Mexican striker a regular goalscorer in Liga MX.
His side sit seventh in the league after an indifferent start which has seen them suffer just one loss, but draw four times.
Penarol are set to travel to Deportivo Anzoategui for their opener.
Elsewhere on the opening match day of the group stages, Botafogo welcome San Lorenzo in Group 2.
Alongside them in the group are Union Espanola and Independiente del Valle, who do battle in Ecuador on February 18.
Atletico Paranaense start their campaign with a clash against The Strongest, while Universitario face Velez Sarsfield also in Group 1.
Group 3 sees Cerro Porteno take on Deportivo Cali and Lanus host O'Higgins.
Carioca 1 leaders Flamengo travel to Leon and Peru Primera Division frontrunners Real Garcilaso host Cruzeiro.
Out-of-form Defensor Sporting will be looking to end a seven-game winless run when they travel to Universidad Chile.
Elsewhere, Nacional do battle with Gremio, Emelec host Bolivar and Atletico Nacional take on Newell's Old Boys.
|Atletico impress in win over Oviedo|
|Herrera sacked by Mexico after ´punch´|
|Gerrard, Keane and Bradley pull out of MLS All-Star squad|
|Marseille reach Diaby agreement|
|Sammer thrilled with Vidal´s winning mentality|
|Platini expected to launch FIFA presidency bid|
|Romanov sacked me over Barca snub - Korobochka|
|Villa complete Crespo capture|
|Wolfsberger v Borussia Dortmund: Bender praises ´fun but exhausting´ training|
|Okaka swaps Sampdoria for Anderlecht|
|Southampton v Vitesse: Fonte excited for ´dream´ sojourn into Europe|
|Bournemouth agree King compensation|
|West Ham v Astra: Kouyate confident West Ham can progress|
|Vidal completes Bayern switch, signs four-year deal|
|Fabregas calls for Champions League improvement|
|BREAKING NEWS: Filipe Luis completes Atletico return|
|Bayern to present Vidal|
|Wenger unfazed by Mourinho´s spending jibe|
|Pellegrini: Sterling will justify price tag|
|Cavani hit with two-match Uruguay ban|
|Bonucci to remain at Juve until 2020|
|No bids for Berahino - West Brom|
|Putin: Blatter deserves a Nobel prize|
|Howe: Swansea a benchmark for Bournemouth|
|New Parma to play in Serie D|
|Mata vows to hit the goal trail at United|
|Drogba ´perfect fit´ for Montreal|
|Berahino ´not yet´ ready for Tottenham - Irvine|
|Romero lauds caring Van Gaal|
|Jese flying at Real under Rafa|
|Sampdoria unwilling to pay €10m for Balotelli|
|Mourinho talks up Arsenal title chances|
|Hull sign Ryan Taylor, Sam Clucas|
|Kane, Pochettino hail MLS All-Star test|
|Di Santo sorry for swift Werder departure|
|Mourinho tells Ronaldo: Team honours come first|
|Khedira returns to Juve training|
|Celtic v Qarabag: Champions League return of utmost importance, says Collins|
|Pellegrini: Jovetic had to go|
|Manquillo completes Marseille move|
|Schalke reject Juve´s Draxler bid|
|Blanc: Di Maria deal ´close´|
|Di Santo debut ends in stalemate|
|Fire sign Drogba, trade him to Montreal Impact|
|FIFA and UEFA welcome ruling on third-party ownership|
|Juve kick off title defence against Udinese|
|Stoke sign free agent Afellay|
|Benitez confident Ramos will stay|
|Fenerbahce issue warning to fans|
|UEFA Champions League Preview: De Boer confident Ajax are ready for Vienna test|
|Bility: I am the favourite for FIFA presidency|
|Pienaar to miss start of season|
|Vietnam 1 Manchester City 8: Sterling shines as tourists run riot|
|All-Star Keane relishing Spurs clash|
|Shaun Wright-Phillips joins brother Bradley at Red Bulls|
|Inter 0 Real Madrid 3: Rodriguez stunner caps comfortable win|
|Villa sign Ayew|
|Watford complete Berghuis transfer|
|Robben returns to Bayern training|
|Pulis anxious to make West Brom additions|
|David Luiz eager to welcome ´fantastic´ Di Maria|
|Fernando injury free ahead of new campaign|
|Hummels blames weight issues for poor season|
|Defoe hoping for early Sunderland goals|
|Depay hints at identity of ´surprise´ United striker|
|Tigres UANL v River Plate: Hosts look to bounce back and make history|
|Crespo set for Villa move|
|Terry: I would never join a Premier League rival|
|Van Gaal coy on United future|
|Romero to cover potential De Gea exit - Van Gaal|
|Uruguay win men´s football gold at Pan Am Games|
|Pirlo thanks New York City fans for ´beautiful´ welcome|
|Mexico capitalised on ´the mistakes we made´ - Schafer|
|Herrera delighted with Mexico display|
|MLS Review: Whitecaps build happy home, Pirlo debuts|
|Guardado wins Golden Ball at Gold Cup|
|NZF confirms Olympics disqualification appeal|
|Jamaica 1 Mexico 3: CONCACAF giants complete form turnaround|
|New York Red Bulls 2 Benfica 1: Another scalp for MLS side|
|Romero signs with United|
|Moghreb Tetouan 1 Al Hilal Omdurman 1: Visitors go top|
|Van Gaal rules out Ronaldo bid|
|Pique open to Barca captaincy|
|Pirlo makes MLS bow in thrilling NYC victory|
|Coutinho and Can return for Liverpool U21s|
|Walcott deal ´closer´, says Wenger|
|Arsenal 1 Wolfsburg 0: Walcott the match-winner in Emirates Cup finale|
|Di Santo links up with Schalke squad|
|Lukaku treble sinks Hearts|
|Benitez again refutes Ronaldo rift rumours|
|Pepe sure Ramos will stay at Real|
|City target De Bruyne named Germany´s Player of the Year|
|Montero spoils McCarthy´s Palace debut|
|Lyon 0 Villarreal 2: Soriano, Baptistao seal comfortable win in Emirates Cup|
|Wijnaldum on target as Newcastle are held|
|Mourinho: Rivals trying to buy the title|
|Jovetic set for Inter medical|
|RFS in ´denial´ over racism issue, claims Football Against Racism in Europe|
|Salah not staying at Chelsea - Mourinho|
|Mbemba closes in on Newcastle switch|
|Ibrahimovic: Di Maria will take PSG to another level|
|Delph: Don´t compare me to Pogba|
|Dundee United sign Sneijder|
|Morata relishing new-look Juve strikeforce|
|Strachan "not scared" of England after World Cup qualifying draw|
|Brazil claim football, basketball, handball gold in Toronto|
|Lampard´s debut delayed again, Pirlo ready to play|
|Mourinho open to Premier League matches in US|
|MLS Review: Texas unites to hurt Galaxy|
|Luis Enrique: United can emulate Barca revival|
|Van Gaal singles out Januzaj, hails United´s forwards|
|Blanc still hopeful PSG will sign Di Maria|
|Irish international Cunningham seeks Bristol City exit|
|Italy embracing Spain challenge in World Cup qualifying|
|Dos Santos brothers in doubt for Gold Cup final|
|PSG 1 Chelsea 1 (aet, 5-6 on penalties): Courtois clinches victory for Mourinho´s men|
|United States 1 Panama 1 (aet, 2-3 on penalties): Mejia denies hosts third|
|ES Setif 1 Al Merreikh 1: Sudanese deny title-holders at home|
|CONCACAF admit referee errors in Gold Cup semi-final|
|Dutch favourites in Group A - Deschamps|
|Barcelona 1 Manchester United 3: Rooney, Lingard and Januzaj maintain winning start|