Chris Coleman should not have to contend with too many injuries for the visit of Croatia, but two key men will definitely miss out.
A red card against Scotland means Aaron Ramsey will be unavailable, while Joe Allen (shoulder) is expected to miss the remainder of the season.
However, Coleman does have some good news as Gareth Bale and David Vaughan are both available.
Igor Stimac will likely make changes to the side that beat Serbia, with veterans Ivica Olic and Josip Simunic both appearing against Sinisa Mihajlovic's side, while the latter is also carrying a knock.
Defender Simunic has been given the all-clear to play, but Stimac may not risk the 35-year-old and instead pick Domagoj Vida, while Olic's replacement will most likely be Nikica Jelavic.
Elsewhere, Niko Kranjcar (knee) is expected to miss out meaning that Josip Radosevic could take his place, or Mateo Kovacic may play in a slightly different role.
If Wales pick up a win on Tuesday, they will be four points behind Croatia with four matches to play.
After the win over Scotland, a victory against the Croatians would see Wales complete back-to-back competitive wins for the first time since Gary Speed's final two matches in charge.
The last time the two sides met, Igor Stimac's side came out on top with a 2-0 win.
Croatia dominated proceedings when they faced Wales in October, maintaining 76 per cent of possession.
Tuesday's match will be only the second time these sides have played each other in competitive competition.
Josip Simunic, Stipe Pletikosa and Darijo Srna could all win their 102nd caps against Wales.
Of the current squad, only Eduardo da Silva with 26 goals has scored more times for Croatia than right-back Srna (pictured), who has 20. The Shakhtar Donetsk man is 11/2 with BetVictor to score at any time in this match.
Sixty per cent of Wales' goals in this qualification campaign have come in the final 15 minutes of matches. Croatia, however, have not scored at all in that section of matches.
|Coach: C. Coleman|
for H. Robson-Kanu 64'
for J. Williams 83'
|Ibrahimovic: United fans are waiting for me|
|Deulofeu shines for Everton, Cook opens Bournemouth account|
|Higuain out to emulate Del Piero|
|Kante does his talking on the pitch - Conte|
|Mourinho explains Fosu-Mensah snub|
|Klopp: No more unwanted players at Liverpool|
|Inter want more than €60m for Icardi - Thohir|
|Leicester can replace Kante - Ranieri|
|Lazio complete Wallace signing|
|Gueye set to join Everton, confirms Di Matteo|
|Tuchel: Transfer market has gone cash crazy|
|Leicester hand King new four-year deal|
|No deal done for Pogba, says Raiola|
|Rooney: Mourinho impact already huge|
|Lafferty hit with FA betting charge|
|Top-four finish never enough for Arsenal, insists Wenger|
|Partey hoping to make his mark on Atletico squad|
|Ibrahimovic in, Schweinsteiger out as United head for Galatasaray friendly|
|Vietto set for Sevilla after completing medical|
|I rejected Besiktas to fight for Barcelona spot, reveals Mathieu|
|De Laurentiis fumes at Higuain´s ´lack of respect´|
|Promising Janssen boosts Tottenham´s title hopes|
|Besiktas sign Adriano on three-year contract|
|Tottenham 0 Atletico Madrid 1: Godin grabs the winner|
|Gomis completes Marseille loan move|
|Iniesta surprised at Casillas-Del Bosque spat|
|New Juve signings do not guarantee success - Allegri|
|New Juve signings do not guarantee success - Allegri|
|Middlesbrough snap up goalkeeper Guzan|
|Guardiola still loves Ribery despite jibes|
|West Ham worry over Tore injury|
|Serie A could be another one-horse race, says Eriksson|
|Schweinsteiger retires from Germany duty|
|Iniesta welcomes Barca´s midfield competition|
|Drogba: No luck in Arsenal scoring record|
|Wenger seeks ´experienced´ striker to bolster Arsenal attack|
|Eriksson and Shanghai SIPG still fighting|
|MLS All-Stars 1 Arsenal 2: Premier League giants win late as Xhaka makes debut|
|Conte: I want aggression and intensity|
|Wimmer backs physical Janssen for Tottenham success|
|Klopp: I wouldn´t spend £100m on one player|
|Sampaoli: Taking Argentina job would be irresponsible|
|Payet absence no excuse - Noble|
|Gabriel Jesus expects Palmeiras exit amid Manchester City rumours|
|Domzale 2 West Ham 1: Crnic double stuns Bilic´s side|
|Juventus target Gabriel Barbosa says Santos exit is close|
|Leicester duo ready for ´once in a lifetime´ Champions League journey|
|Fabregas apologises for Klavan lunge|
|Reschke: Guardiola wanted Tuchel to be his successor|
|Pochettino expecting further Tottenham signings|
|Given retires from international football|
|Abdi and Pudil swap Watford for Wednesday|
|Leicester sign Chilwell to new five-year deal|
|Klopp pre-season ´most intense ever´ for Sturridge|
|Simeone backs Griezmann for Ballon d´Or final three|
|Puskas Award winner quits football - to become a gamer|
|Simeone: Champions League final felt like a death|
|Higuain: I have to prove my worth to Juventus|
|Guardiola confirms interest in Sane and Stones|
|Moreno set for Manchester City switch and Deportivo loan, claims agent|
|De Laurentiis pushed me into Juventus move - Higuain|
|Savic seeking to put injury woe behind him|
|Borussia Dortmund 1 Manchester City 1 (5-6 pens): Gunn´s shootout saves earn Guardiola first win|
|Oblak: Atleti need consistency and luck|
|Warburton rubbishes Van Persie rumours|
|Hospitalised Grujic ´can´t remember anything´, says Klopp|
|Premier League, Serie A appeal to Simeone|
|Copa Libertadores success ´a dream´ for Borja|
|Pochettino honoured by Simeone comparisons|
|Argentina have not asked for Sampaoli - Monchi|
|Ancelotti: Fitness the key for Bayern|
|Bayern win ´a small foundation´ for Montella´s Milan|
|Icardi missing due to injury - Mancini|
|Emery satisfied with warm-up win over Madrid|
|Conte quiet on Tevez, apologises for Fabregas red|
|´It was far from a friendly´ - Klopp on Chelsea defeat|
|Madrid off the pace, concedes Zidane|
|Chelsea 1 Liverpool 0: Cahill´s header seals win|
|Grujic says Chelsea´s Matic told him to sign for Liverpool|
|Bolasie wants to see Berahino and Benteke at Crystal Palace|
|Rodgers hails ´outstanding result´ as Celtic draw|
|Walcott claims level of MLS is close to Premier League|
|Bayern Munich 3 AC Milan 3 (3-5 on penalties): Bonaventura seals win|
|Atletico Nacional 1 Independiente del Valle 0 (2-1 agg): Borja secures Copa Libertadores crown|
|Icardi absent again as Inter held by Estudiantes|
|Real Madrid 1 Paris Saint-Germain 3: Zidane oversees poor pre-season start|
|Hazard vows to rediscover best Chelsea form|
|Klopp reveals why he sent Sakho back to Liverpool|
|Tevez: Conte wanted me at Chelsea|
|Sunderland hopeful Defoe will be fit for Man City opener|
|Sundowns overcome Zamalek to book semi-final spot|