Bayern Munich will start their campaign to retain their DFB-Pokal crown against minnows Schwarz-Weiss Rehden on Monday.
Bayern wrapped up an historic treble with a 3-2 victory over Stuttgart in last season's final.
Jupp Heynckes was in charge for that success, however former Barcelona manager Pep Guardiola has since taken the reins, and he will be looking for his side to produce a professional performance against the fourth tier side.
Stuttgart begin their campaign against Dynamo Berlin on Sunday, while Juergen Klopp's Borussia Dortmund will be hoping to avoid a shock against Wilhelmshaven on Saturday.
Goalkeeper Roman Weidenfeller and midfielders Sven Bender, Jakub Blaszczykowski and Henrikh Mkhitaryan are all expected to miss out, but Klopp's side will still be expected to come through with little difficulty.
Schalke, who have won the competition on five occasions, face fifth-tier club Nottingen and will undoubtedly be aiming to improve on their run to the third round last season.
Three-time winners Hamburg fell in the first round against Karlsruher last term, and manager Thorsten Fink is keen to avoid the same fate this time around Schott Jena on Sunday.
"Obviously as a Bundesliga side we are the clear pre-match favourites. Still, the same rule applies every year: You must not underestimate any opponent in the DFB-Pokal," he told the club's official website.
"My team look solid and focused, so that won't happen to us. The lads should be totally looking forward to Sunday after the long break and go out there and enjoy it.
"The necessary aggression will automatically come. That way we will live up to our billing as pre-match favourites and progress to round two."
|Coach: P. Uzelac|
for Y. Chaib 65'
for A. Neumann 72'
for K. Artmann 89'
|Bournemouth beaten by battling Cardiff|
|Immobile injury overshadows Sevilla win at Vicarage Road|
|Lambert at the double on West Brom bow|
|Real sign up Spain starlet Vallejo|
|Valencia blow for West Ham|
|Elche cleared to play in Segunda Division|
|Baker pens a new Villa deal|
|Lambert makes Albion switch|
|Gillingham and Scally found guilty of ´race victimisation´|
|Inter complete Jovetic signing|
|Arsenal v Chelsea: Mourinho cools Wenger rivalry ahead of Wembley meeting|
|Villa snap up Gestede|
|Villa land Veretout|
|Mourinho coy on fresh Stones bid|
|Aubameyang commits to Dortmund|
|Torres on the hunt for trophies with Atletico|
|Cazorla and Walcott sign new Arsenal deals|
|Quick start vital in Arsenal title bid - Wilshere|
|I have received no offers - Guardiola|
|Barcelona to monitor Turan injury|
|Zorc ´surprised´ by Grosskreutz-Tuchel row|
|Phil Neville could be England manager - Moyes|
|UEFA to investigate Shakhtar´s Fred selection|
|City loan Unal to Genk|
|China v South Korea: Stielike looking for new options|
|North Korea v Japan: Halilhodzic to unearth new faces|
|Drogba: I want to be ready, not a burden|
|Hamstrung Clasie in doubt for Southampton league opener|
|West Ham goalscorer Zarate bemoans Astra comeback|
|Negredo quashes Valencia exit rumours|
|´This is not my Sampdoria´ - president Ferrero|
|Criscito could join Inter - agent|
|CBF endorse Zico´s FIFA presidency campaign|
|Hawit to handle CONCACAF´s referee review|
|Falcao is a ´top, top player´ - Terry|
|Tuchel pleased by Dortmund fighting qualities|
|Premier League rules out schedule changes for Champions League clubs|
|UEFA Europa League Review: Southampton stroll as Sampdoria are humiliated|
|Koeman confident of Southampton progress|
|Bilic frustrated with Collins´ red card, Valencia injury|
|Bamford hits hat-trick in Bromley friendly|
|Southampton 3 Vitesse 0: Play-offs in sight for Koeman’s men|
|Wolfsberger 0 Borussia Dortmund 1: Tuchel begins tenure with narrow win|
|West Ham 2 Astra 2: Collins red allows visitors back in|
|Kukesi - Legia match abandoned|
|Joining Roma an ´easy decision´ - Szczesny|
|Chiriches joins Napoli from Spurs|
|Mihajlovic pleased with Milan improvements|
|Injured Chiellini to miss Supercoppa|
|Newcastle complete Mbemba swoop|
|Blackpool protest incurs FA fine|
|Scottish Premiership Preview: Dons seek to run Celtic closer|
|Paris Saint-Germain v Lyon: Ibrahimovic ready for season opener|
|Wolfsburg v Bayern Munich: De Bruyne centre of attention|
|Real Madrid 0 Milan 0 (10-9 on pens): Real lift second International Champions Cup|
|De Bruyne a doubt for Supercup clash|
|Reine-Adelaide needs time to blossom, says Walcott|
|Rodgers will not rush Ibe development|
|Napoli not interested in selling Higuain - De Laurentiis|
|Wilson agrees Bournemouth extension|
|Messi makes early Barcelona return|
|Ibrahimovic: My next move will be a very big surprise|
|Van Aanholt backs Lens to shine|
|Besiktas complete loan move for Gomez|
|McClaren: No decision on Coloccini future|
|Kane, Kaka hail goalkeeper Rimando|
|Chung eager to become FIFA president|
|Yedlin open to loan move|
|Tigres not scared of River trip - Ferretti|
|MLS Preview: Wright-Phillips, Lampard set to debut|
|´A draw is good for us´ - River´s Gallardo confident of Libertadores success|
|Di Maria poised for PSG switch - Blanc|
|Franjic: Bring on Bangladesh, Tajikistan|
|Van Gaal bemoans De Gea situation|
|MLS All-Stars 2 Tottenham 1: Kane stunner can´t spare Spurs|
|Manchester United 0 Paris Saint-Germain 2: Van Gaal´s defensive frailties exposed|
|Tigres UANL 0 River Plate 0: Visitors lose Gallardo in Copa Libertadores final first leg|
|Penedo parts ways with Galaxy|
|Benitez backs Mihajlovic, Milan to succeed|
|Barnetta joins Union in MLS|
|Allegri critical of Morata´s attitude|
|Franjic targets more A-League glory with City|
|Beto undaunted by Super Cup clash against Barca|
|Advocaat upbeat despite another Sunderland flop|
|McClaren praises Newcastle recruit Mitrovic|
|Payet will improve West Ham - Bilic|
|Pulis confirms interest in Lambert|
|Deila expects tough test in Baku|
|Drogba mobbed by Montreal Impact fans|